RAY-AAA98I Binary Gradient Pump

Main features:
This configuration is suitable for feed, food , chemical, pharmaceutical and other amino acid analysis, using pre-column derivatization method detachable 21 kinds or 18 kinds of amino acids. Can also be used as a binary HPLC .

Technical parameters:

Gradient Pump: Flow rate 0.001–10 ml/min; Accuracy ±0.2%.

Gradient Accuracy: ≤0.06% (ASTM).

Max Pressure: 9000 psi.

Degassing System: Built-in 4-channel vacuum degasser.

Additional Functions: Auto plunger rod cleaning, anti-salt precipitation.

Sampling: Loop and variable sampling, 1–500 µl injection volume, Reproducibility <1% @10 µl.

Sample Tray: 2 trays, 48 positions each (1.5 ml bottles).

Memory Effect: <0.01%.

Chromatographic Column: Sodium ion exchange (4×150 mm).

Derivatization: Ninhydrin post-column method.

Temperature Control: RT–150°C, 0.1°C increments.

Spectroscopy: Aberration concave diffraction grating, 190–800 nm range.

Environment: 10–30°C, ≤80% RH.

(1) P98-Ⅰ type binary gradient infusion pump
Flow range : 0.01ml / min ~ 15ml / min
Flow rate accuracy : <3μl (0.01ml / min ~ 0.1ml / min), <1% (0.5ml / min ~ 9.99ml / min)
B liquid % Accuracy : = ± 0.5% ABS
Pressure range : 0.00MPa ~ 42MPa (420Kgf / cm2)
Microprocessor controlled proportional valve control encoder disk ; high accuracy.
Imported motor drive imports , subdivision twenty thousand steps, no mechanical noise ; to ensure accuracy.

Imported sealing systems , high pressure capability .
Single- piston reciprocating pump , a single one-way valve design solution without degassing .
Imported high pulsation damper , buffering capacity, so that the minimum ripple .
Multiple time program , do a gradient .
Automatic memory program , easy to bring up the methods.
Display: LCD screen display.
Display mode : the actual pressure , flow rate setting , the pressure lower limit .
Security Control : The pressure limit control , buzzer alarm, automatic shutdown.
Vertical chassis , touch panel.

(2) UV98-Ⅰ variable wavelength UV-Vis detector
Optical system : Imported grating monochromator design , imported photoelectric receiving device .
Light source: deuterium lamp Hamamatsu L6302
Wavelength range : 190 ~ 700nm
Wavelength accuracy : ± 1nm
Wavelength repeatability : ± 0.4nm
Noise : 2.5 × 10-5AU (pp) ( dynamic index , the mobile phase of methanol , 254nm, next column )
Drift : 1 × 10-3AU / hr ( dynamic index , the mobile phase of methanol , 254nm, next column )
Attenuation range : 0.002 ~ 2AU / mV 10 files

Detection cell volume : 4.5μl;
Detection cell voltage : 120 Kgf / cm2 ( patent pool structure design )
Minimum detectable amount : 2 × 10-8g / ml ( naphthalene / methanol solution )
Microprocessor-controlled , automatic scanning of stay ; wavelength repeatability.
LCD screen display, automatically find the wavelength , automatic zero adjustment.
Unique deuterium lamp control , striking voltage make deuterium lamp life up to 2500 hours.
Patented back pressure design, the bubble is not easy to enter the flow cell.
Vertical chassis , touch panel ; simple hydraulic connections .
Main features:
This configuration is suitable for feed, food , chemical, pharmaceutical and other amino acid analysis, using pre-column derivatization method detachable 21 kinds or 18 kinds of amino acids. Also available as two yuan HPLC

1.    SPECIFICATION

a. Operating pressure: 0～42MPa (6000 psi) maximum.

b. Flow rate range: 0.1～15ml/min；

c. Flow rate precision: ±1%；

d. Flow rate accuracy: 25ml/368～387s(±3%)；

e. Compositional precision:0.5% absolute

f. Pressure solution: 0.1MPa；

g. Over pressure protection: pump shuts off if pressure exceeds limit specified by user-42.0 Mpa

(6000 psi) maximum.

2.Environment

ａ.Temperature requirements: ambient temperature range 10 to 35℃

ｂ. Operates in relative humidity from 5% to 80%;

ｃ. Power requirements: 220Vac±5%,

ｄ.Power frequency: 50±0.5Hz (voltage and frequency specified on the tag attached to the power cord)

3. GENERAL PARAMETERS

a. Power Supply: 400W；

b. Dimensions: 450mm(D)×210mm(W)×390mm(H)；

c. Weight: 20kg；

UV98I UV Detector

1. Specification

Wavelength range：190-700nm

Spectral bandwidth：5nm

Wavelength accuracy：±2nm

Wavelength reproducibility：±0.2nm

Absorbance range：

Recorder output：0.002au/mV to 2au/mV in steps of 1, 2, 5

Data system output：2au/V, fixed

Noise：5×10^-5au (Peak-Peak)

Test Conditions： Wavelength: 254nm

Mobile phase: methanol

Flow rate: 1ml/min

Time constants: 0.5s

Drift：1×10^-3au/hr

Test Conditions： Wavelength: 254nm

Mobile phase: methanol

Flow rate: 1ml/min

Time constants: 0.5s

Flow cell volume：4.5μl

Time constants：0.05s, 0.5s, 5s

Autozero:Yes

2.  General Parameters

a. Power Supply: 200W；

b. Dimensions: 450mm(D)×180mm(W)×390mm(H)；

c. Weight: 15kg；

Figure 1  18 Amino Acid Stand Sample

1. Asp  2.Glu  3. Ser  4. Gly   5 His  6. Arg   7. Thr  8. Ala  9. Pro  11. Val

12. Met  13. (Cys)₂  14. Ile  15. Leu  16. Trp  17. Phe  18. Lys